Use the following structures of amino acids to answer the

Use the following structures of amino acids to answer the questions below. Note that the difference in the structures (the side chains) is highlighted by gray shading.

a) A student performed chromatography of the four amino acids and the results were shown in the chromatogram below. If an anion exchange column (column is positively charged) was used in a neutral buffer, assign each amino acid to the corresponding peak in the chromatogram. (4 points)

b) Write 2 – 3 sentences to describe the interactions that will occur for each amino acid with the anion exchange column and its effect on column retention.

c) Write 2 – 3 sentences to describe how the results would differ if a cation exchange column (negatively charged column) were used.