1. What are the IMViC test and its significance? 2.

1. What are the IMViC test and its significance?

2. List 2 enzymes for citrate utilization and their role for citrate positive bacteria.  Identify the medium used for the citrate test and its composition. 

3. What‘s the indole test? Name the specific enzyme bacteria uses to breakdown tryptophan amino acid.

4. Identify the specific reagent, ……………………………………  used to detect bacteria’s ability to utilize tryptophan.  Write the specific role of each component of this reagent.

5. List components of the indole medium.  What’s the source of peptones in the medium.

6. What’s the butanediol pathway and how it is detected in bacteria?

7. Write a biochemical basis for the detection of the butanediol pathway. Use the back of this page. Include composition of VP-I and VP – II reagents 

8. What is the MR test?

9.Write the result of the MR – VP test ( on the back ) and explain the result

 

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